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(A-B) H&E staining of primary TC tissue and corresponding 3DP organoids.Scale bars: 50 μm (C) Immunofluorescence images of TC organoids stained for DAPI, CD56, <t>CD117,</t> and pan-CK. Scale bars: 50 μm (D-E) H&E staining of primary THYM tissue and corresponding 3DP organoids. Scale bars: 50 μm (F) Immunofluorescence images of THYM organoids stained for DAPI, P63, pan-CK, and TdT.Scale bars: 100 μm. (G) CNV profiles of TC-Tumor. (H)TC-3DP organoids (I) TC-Matrigel organoids. (J) CNV profiles of THYM-Tumor. (K) THYM-3DP organoids. (L)THYM-Matrigel organoids.
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(A-B) H&E staining of primary TC tissue and corresponding 3DP organoids.Scale bars: 50 μm (C) Immunofluorescence images of TC organoids stained for DAPI, CD56, CD117, and pan-CK. Scale bars: 50 μm (D-E) H&E staining of primary THYM tissue and corresponding 3DP organoids. Scale bars: 50 μm (F) Immunofluorescence images of THYM organoids stained for DAPI, P63, pan-CK, and TdT.Scale bars: 100 μm. (G) CNV profiles of TC-Tumor. (H)TC-3DP organoids (I) TC-Matrigel organoids. (J) CNV profiles of THYM-Tumor. (K) THYM-3DP organoids. (L)THYM-Matrigel organoids.

Journal: Materials Today Bio

Article Title: Biomimetic 3D-Bioprinted organoids of thymic epithelial tumors for translational drug screening and biomarker identification

doi: 10.1016/j.mtbio.2026.102878

Figure Lengend Snippet: (A-B) H&E staining of primary TC tissue and corresponding 3DP organoids.Scale bars: 50 μm (C) Immunofluorescence images of TC organoids stained for DAPI, CD56, CD117, and pan-CK. Scale bars: 50 μm (D-E) H&E staining of primary THYM tissue and corresponding 3DP organoids. Scale bars: 50 μm (F) Immunofluorescence images of THYM organoids stained for DAPI, P63, pan-CK, and TdT.Scale bars: 100 μm. (G) CNV profiles of TC-Tumor. (H)TC-3DP organoids (I) TC-Matrigel organoids. (J) CNV profiles of THYM-Tumor. (K) THYM-3DP organoids. (L)THYM-Matrigel organoids.

Article Snippet: The following primary antibodies were used: Pan-CK (# MA513203 , invitrogen) at a dilution of 1:100, CD117 (AF1356-SP, R&D system) at a dilution of 1:100, CD56/NACM1 (#ab313779, abcam) at a dilution of 1:100, p63 (#ab124762, abcam) at a dilution of 1:100, TdT (#TB4932S, abmart) at a dilution of 1:100, and Anti-gamma H2A.X (phospho S139) antibody (#ab2893, abcam) at a dilution of 1:100.

Techniques: Staining, Immunofluorescence